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Nucleic Acids Research, 1983, Vol. 11, No. 13 4417-4434
© 1983

MOLECULAR BIOLOGY

DNase I cleavage of adenoviral nucleoprotein

Martha J. Fedor* and Ellen Daniell

Department of Molecular Biology and Virus Laboratory, University of California Berkeley, CA 94720, USA

Received March 14, 1983. Revised June 8, 1983. Accepted June 8, 1983.

Cleavage products resulting from DNase I treatment of adenoviral nucleoprotein were examined by gel electrophoresis, Southern blotting and hybridization to cloned restriction fragments derived from various regions of the viral genome. DNase I produced specific double-stranded cleavages in DNA of purified adenoviral cores and in DNA of intranuclear viral chromatin at early and late times of infection. At least some of these sites were also cleaved by DNase I in purified viral DNA, showing that sequence specificity of DNase I cleavage may contribute to the observation of specific double-stranded DNase I cleavage sites in adenoviral nucleoprotein. In addition, sites were observed which were specific either for cores or for intranuclear chromatin. In contrast to many cellular genes which have been characterized, there was no obvious relationship between DNase I cleavage sites and other features of the viral genome such as promoters or polyadenylation sites.

*Current Address: Department of Structural Biology, School of Medicine, Stanford University, Stanford, CA 94305, USA


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