Nucleic Acids Research, 1983, Vol. 11, No. 13 4435-4451
© 1983
MOLECULAR BIOLOGY |
Biogenesis of mitochondria: the mitochondrial gene (aap1) coding for mitochondrial ATPase subunit 8 in Saccharomyces cerevisiae
Department of Biochemistry, Monash University Clayton, Victoria 3168, Australia
*To whom correspondence should be sent
Received May 20, 1983. Accepted June 13, 1983.
A mitochondrial gene (denoted aap1) in Sacchaaromyces cerevisiae has been characterized by nucleotide sequence analysis of a region of mtDNA between the oxi3 and oli2 genes. The reading frame of the aap1 gene specifies a hydrophobic polypeptide containing 48 amino acids. The functional nature of this reading frame was established by sequence analysis of a series of mit– mutants and revertants. Evidence is presented that the app1 gene codes for a mitochondrially synthesized polypeptide associated with the mitochondrial ATPase complex. This polypeptide (denoted subunit 8) is a proteolipid whose size has been previously assumed to be 10 kilodaltons based on its mobility on SDS-polyacrylamide gels, but the sequence of the aap1 gene predicts a molecular weight of 5,815 for this protein.
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