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Nucleic Acids Research, 1983, Vol. 11, No. 13 4453-4466
© 1983

CHEMISTRY

Spectroscopic studies of (m5dC-dG)3: thermal stability of B- and Z-forms

Brigitte Hartmann1, Nguyen Thanh Thuong1, Jean Pouyet2, Marius Ptak1 and Marc Leng1

1Centre de Biophysique Moléculaire, CNRS 1A, avenue de la Recherche Scientifique, 45045 Orleans Cedex 2Institut de Biologie Moléculaire et Cellulaire 15, rue Descartes, 67084 Strasbourg Cedex, France

Received May 19, 1983. Revised June 15, 1983. Accepted June 15, 1983.

The hexanucleoside pentaphosphate d(m5CpGpm5CpGpm5CpG) has been studied in solution by ultra-violet absorption, circular dichroism and 31P nuclear magnetic resonance under various experimental conditions. In 0.2 M NaClO4 at low temperature, an hexamer duplex is formed which has a B or B-like conformation. As the salt concentration is increased, a transition from a B-form to the Z-form occurs and is complete in 3 M NaClO4. In 3 M NaClO4, the behavior of the Z double helix is complex as a function of temperature. The variation of the circular dichroism at 295 nm is biphasic. A first transition occurs over a large range of temperature and corresponds to a conformational change due to a non-cooperative intramolecular process. Ultra-violet absorption and 31P nuclear magnetic resonance show that the new conformation arising from a distortion of the backbone is not similar to that observed in low salt conditions (B-form). At high hexanucleotide concentration, aggregates are formed. The second transition is cooperative and corresponds to the melting of a double stranded helix into single strands.


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