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Nucleic Acids Research, 1983, Vol. 11, No. 13 4569-4582
© 1983

CHEMISTRY

Conformationsl analysis of the trinucleoside diphosphate 3'd(A2'-5'A2'-5'A). An NMR and CD study*

Johannes Doornbos, Ramamurthy Charubala1, Wolfgang Pfleiderer1 and Cornelis Altona

1Fakultät für Chemie, Universität Konstanz Postfach 5560, D-7750 Konstanz-1, FRG Gorlaeus Laboratories, Department of Chemistry, State University Leiden P.O.Box 9502, NL-2300 RA Leiden, The Netherlands

Received April 12, 1983. Accepted May 2, 1983.

A 500 MHz and 300 MHz NMR study of the trinucleoside diphosphate 3'd(A2'-5'A2'-5'A) is presented. In addition, circular dichroism is used to study base stacking in the title compound. The complete 1H-NMR spectral assignment of the sugar ring proton signals is given. Information about the sugar ring (N- or S-type conformation) and about the backbone geometry along C4'-C5' and C5'-O5' bonds is obtained from the NMR coupling constants. It is shown that the trimer mainly occurs in the N-N-N stacked state at low temperatures; the presence of a minor amount of N-N-S conformational sequence is indicated.

*This paper is no.32 in the series ‘Nucleic Acids Constituents’. For no.31 see previous paper


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