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Nucleic Acids Research, 1983, Vol. 11, No. 14 4677-4688
© 1983

MOLECULAR BIOLOGY

Inducible high level synthesis of mature human fibroblast interferon in Escherichia coli

Erik Remaut, Patrick Stanssens and Walter Fiers

Laboratory of Molecular Biology, State University of Ghent Ledeganckstraat 35, 9000 Ghent, Belgium

Received June 7, 1983. Accepted June 27, 1983.

We have obtained high level synthesis in Escherichia coli of mature human fibroblast interferon using a plasmid vector that was designed to allow easy coupling of a DNA coding region to the initiator AUG of the replicase gene of the RNA phage MS2 cloned downstream of phage {lambda} 's leftward promoter. The activity of the promoter can be regulated by temperature. Induced cells accumulated the interferon up to 4% of the total cellular protein. The biological activity of the product amounted to 4 x 109 international units per litre of culture. The synthesis of human fibroblast interferon was shown to drastically inhibit the growth rate of the bacterial host.


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