Skip Navigation

This Article
Right arrow Print PDF (1590K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (53)
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Groner, B.
Right arrow Articles by Ponta, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Groner, B.
Right arrow Articles by Ponta, H.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1983, Vol. 11, No. 14 4713-4725
© 1983

MOLECULAR BIOLOGY

Transcription initiation of transfected mouse mammary tumor virus LTR DNA is regulated by glucocorticoid hormones

Bernd Groner, Nancy E. Hynes, Ursula Rahmsdorf and Helmut Ponta

Kernforschungszentrum Karlsruhe, Institut fur Genetik und Toxikologie Postfach 3640, D-7500 Karlsruhe 1, FRG

Received May 20, 1983. Accepted June 24, 1983.

A chimeric gene, recombined in vitro, containing a long terminal repeat (LTR) sequence from the proviral DNA of mouse mammary tumor virus and the thymidine kinase (tk) gene of Herpes Simplex Virus was introduced into L tk– cells. No transcription of LTR RNA was observed in transfected cells when glucocorticoid hormones were absent from the growth medium. Accumulation of LTR initiated RNA was measured upon hormone addition by the single strand specific nuclease RNA mapping procedure. The accumulation was rapid (detectable after 7.5 minutes), independent of simultaneous protein synthesis and mediated by a functional glucocorticoid receptor complex. Glucocorticoid hormones affect LTR transcription at the level of initiation. The rate of initiation (1.8x10–2 molecules/cell/sec) and a half life of about 30 minutes could be calculated for LTR RNA. The half life of LTR RNA is independent of the presence of hormone.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Virol.Home page
F. Rouault, S. B. Nejad Asl, S. Rungaldier, E. Fuchs, B. Salmons, and W. H. Gunzburg
Promoter Complex in the Central Part of the Mouse Mammary Tumor Virus Long Terminal Repeat
J. Virol., November 15, 2007; 81(22): 12572 - 12581.
[Abstract] [Full Text] [PDF]

Home page
 Pharmacol. Rev.Home page
E. Falkenstein, H.-C. Tillmann, M. Christ, M. Feuring, and M. Wehling
Multiple Actions of Steroid Hormones---A Focus on Rapid, Nongenomic Effects
Pharmacol. Rev., December 1, 2000; 52(4): 513 - 556.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Endocrinol. Metab.Home page
M. Wehling, C. H. Spes, N. Win, C. P. Janson, B. M. W. Schmidt, K. Theisen, and M. Christ
Rapid Cardiovascular Action of Aldosterone in Man
J. Clin. Endocrinol. Metab., October 1, 1998; 83(10): 3517 - 3522.
[Abstract] [Full Text]

Home page
 HypertensionHome page
S. Murasawa, H. Matsubara, K. Kizima, K. Maruyama, Y. Mori, and M. Inada
Glucocorticoids Regulate V1a Vasopressin Receptor Expression by Increasing mRNA Stability in Vascular Smooth Muscle Cells
Hypertension, October 1, 1995; 26(4): 665 - 669.
[Abstract] [Full Text]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.