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Nucleic Acids Research, 1983, Vol. 11, No. 14 4809-4821
© 1983

MOLECULAR BIOLOGY

Isolation and characterization of genomic moose DNA clones containing sequences homologous to tRNAs and 5S rRNA

James C. Hu+, Babette D. Cote, Elsebet Lund and James E. Dahlberg

Department of Physiological Chemistry, University of Wisconsin-Madison Madison, WI 53706, USA

Received April 11, 1983. Accepted June 23, 1983.

We have cloned and characterized three fragments of Balb/c mouse DNA which hybridize to mouse cell tRNAs. Fractionation of the tRNAs which hybridize to these clones reveals that two of the clones, {lambda}Mt-4A and {lambda}Mt-6A hybridize to only one or two tRNAs, while one clone, {lambda}Mt-4B, hybridizes to at least seven tRNAs. Two of the tRNAs were identified as Formula and Formula, and others have been identified as tRNAs which are selectively encapsidated into virions of rourine leukemia virus and avian reticuloendotheliosis virus. The DNA sequences of putative genes for Formula and Formula, plus flanking regions, were determined. A clone of Balb/c mouse DNA which selectively hybridized to 5S rRNA was also isolated and partially characterized.

+Present address: Department of Bacteriology, University of Wisconsin, Madison, WI 53706, USA


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