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Nucleic Acids Research, 1983, Vol. 11, No. 15 5021-5036
© 1983

MOLECULAR BIOLOGY

Isolation and characterisation of genomic and cDNA clones for an androgen-regulated secretory protein of rat seminal vesicles

Lindsay Williams, Charles McDonald, Stephen Jackson, Elizabeth McIntosh and Stephen Higgins*

Department of Biochemistry, University of Leeds Leeds LS2 9JT, UK

*To whom correspondence should be addressed.

Received June 23, 1983. Accepted July 13, 1983.

Testosterone controls the synthesis of seminal vesicle protein F in male rats by regulating the cellular concentration of its mRNA (mRNAF). Phage {lambda} recombinants have been isolated containing the complete F gene. In addition plasmids have been constructed containing cDNAF sequences some of which are probably full-length ({bsim}700 bp). Detailed restriction mapping shows that the F gene is 1.7 kbp long and contains {bsim}1.0 kbp of intervening sequencearranged in at least two introns (420 bp and 600 bp). Part of cDNAF has been sequenced showing that the terminal125 bp of the 3' untranslated region of mHNAF has substantial (>70%) sequence homology with the 3'end of the mRNA coding for another androgen-dependent seminal vesicle protein (protein S). The cloned F gene has been detected in liver and seminal vesicle DNA along with an homologous but structurally different gene. The hormonal control of mRNAF was examined with cDNAF. A pronounced ({bsim}3000-fold) differential response to testosterone was observed.


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