Nucleic Acids Research, 1983, Vol. 11, No. 15 5147-5158
© 1983
MOLECULAR BIOLOGY |
The nucleotide sequence of the trimethoprim-resistant dihydrofolate reductase gene harbored by Tn7
Wellcome Research Laboratories Research Triangle Park, NC 27709, USA
Received April 29, 1983. Revised June 30, 1983. Accepted June 30, 1983.
The complete nucleotide sequence of the type I dihydrofolate reductase gene from Tn7 was determined. The structural gene coded for a polypeptide of 157 amino acid residues. The polypeptide deduced from the DNA sequence had a molecular weight of 17,577 which was in good agreement with that estimated by mobility in SDS-polyacrylamlde gels. Sequences were identified proximal to the coding region which were similar to those found in the consensus E. coll promoter region and for the initiation of protein synthesis. Features consistent with the termination of RNA transcription were present distal to the structural gene. No homology was apparent when the DNA sequence of the type I gene was compared to the sequence of the type II plasmid DHFR genes, but sequence homology was evident when the type I and E. coli chromosomal enzymes were compared. Homology was greatest in the regions coding for amino acids which in the E. coli chromosomal enzyme are associated with substrate, cofactor and inhibitor binding.
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