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Nucleic Acids Research, 1983, Vol. 11, No. 17 5795-5810
© 1983

MOLECULAR BIOLOGY

In vivo regulation of the uvrA gene: role of the "–10" and "–35" promoter regions

Claude Backendorf*, Jourica Brandsma, Tonja Kartasova and Pieter van de Putte

Department of Molecular Genetics, State University of Leiden 2333 AL Leiden, The Netherlands

Received August 1, 1983. Accepted August 11, 1983.

The effect of increasing deletions in the uvrA promoter region on the transcriptional efficiency was quantitatively analysed by fusion to the galK structural gene. A physical analysis of uvrA messenger RNA synthesis from the different deletion plasmids was performed using the S1 mapping technique. Both methods indicate that the uvrA "–10" promoter sequence is sufficient to trigger uvrA transcription. Although not essential, the "–35" region, which is overlapping with the LexA binding site, is shown to have an enhancing function, as the exposure of this region after SOS induction results in a 3- to 4-fold increase in uvrA transcription. A model is presented which accounts both for the oberved basal and induced expression of the uvrA gene on a molecular level.

* In the case of uvrA two theoretical "–35 sequence" can be indicated. The sequence 5' TATTCA 3' is probably the best candidate as it is separated by only 17 nucleotides from the "–10 sequence".


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