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Nucleic Acids Research, 1983, Vol. 11, No. 17 5811-5819
© 1983

MOLECULAR BIOLOGY

Sequence of human haptoglobin cDNA: evidence that the {alpha} and ß subunits are coded by the same mRNA

G. Raugei, G. Bensi, V. Colantuoni1, V. Romano2, C. Santoro3, F. Costanzo1 and R. Cortese

European Molecular Biology Laboratory Meyerhofstrasse 1, 6900 Heidelberg, FRG 1On leave of absence from Istituto di Chimica Biologica, II Facolta di Medicina, University of Naples Nsples, Italy 2On leave of absence from Istituto di Biologia dello Sviluppo, Consiglio Nazionale defle Ricerche Palermo, Italy 3On leave of absence from Istituto di Biologia, Facolta di Medicina, University of Turin Turin, Italy

Received July 22, 1983. Accepted August 5, 1983.

We have isolated and sequenced a cDNA clone coding for human haptoglobin. Our sequence shows that haptoglobin is very likely synthesized as a single polypeptide chain which is then cleaved at an Axg residue to generate its two characteristic {alpha} and ß subunits. Southern blot analysis suggests that there are at least two copies of the haptoglobin gene per haploid genome.


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