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Nucleic Acids Research, 1983, Vol. 11, No. 17 5821-5835
© 1983

MOLECULAR BIOLOGY

CAP binding proteins associated with the nucleus

E. Patzelt, D. Blaas and E. Kuechler

Institut für Biochemie Währingerstraße 17, A-1090, Vienna, Austria

Received July 20, 1983. Accepted August 12, 1983.

Cap binding proteins of HeLa cells were identified by photo-affinity labelling using the cap analogue {gamma}–[32P]-[4-(benzoyl-phenyl) methylamido]-7-methylguanosine-51-triphosphate. Photo-reaction with whole cell homogenates resulted in specific labelling of five major polypeptides. The small molecular weight polypeptideappeared to be identical to the 24 000 to 26 000 dalton cap binding protein previously identified in initiation factors. A cap binding protein of 37 000 dalton was found in initiation factors as well as in preparations of crude nuclei. It was released from nuclei by washing with buffer of moderate salt concentration. Three high molecular weight cap binding proteins (~120 000,~89 000,~80 000 dalton) were found in the nuclear fraction and were only partly released upon nuclease digestion and high salt extraction.


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