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Nucleic Acids Research, 1983, Vol. 11, No. 17 5855-5864
© 1983

MOLECULAR BIOLOGY

Essential structure of E. coli promoter effect of spacer length between the two consensus sequences on promoter function

Takashi Aoyama*,+, Mituru Takanami*, Eiko Ohtsuka§, Yoshio Taniyama§, Ryuji Marumoto§, Hiroko Sato§ and Mono Ikehara§

*Institute for Chemical Research, Kyoto University Uki, Kyoto 611 §Faculty of Pharmaceutical Sciences, Osaka University Osaka 565, Japan

+To whom correspondence should be addressed

Received July 12, 1983. Accepted August 9, 1983.

A promoter with the consensus sequences(TTGACA and TATAAT) at –35 and –10 regions was constructed, and the distance between the two consensus sequences was independently altered at two restriction sites. In an in vitro transcription system, a maximum activity was observed at the spacer length of 17 base-pairs regardless of the sites of space adjustment. Evidence was also provided that the start point of transcription was fixed by the consensus sequence at the –10 region.


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