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Nucleic Acids Research, 1983, Vol. 11, No. 17 5877-5892
© 1983

MOLECULAR BIOLOGY

Nucleotide sequence of a Euglena gracilis chloroplast genome region coding for the elongation factor Tu; evidence for a spliced mRNA

Paul-Etienne Montandon and Erhard Stutz

Laboratoire de Biochimie, Université de Neuchâtel Ch. de Chantemerle 18, CH-2000 Neuchâtel, Switzerland

Received July 12, 1983. Accepted August 12, 1983.

We characterize a 1.95 kb transcription product of the Euglena gracilis chloroplast DNA fragment Eco-N + Q by SI nuclease analysis and DNA sequencing and show that it is the product of three splicing events. Exon 1 (0.45 kb), exon 2 (0.74 kb) and 175 nucleotides of exon 3 (0.53 kb) code for the chloroplast elongation factor protein (EF-Tu). The remaining part of exon 3 and exon 4 (0.23 kb) have unidentified open reading frames. The chloroplast EF-Tu protein has 408 aminoacids and is to 70percnt; homologous with the E. coli EF-Tu protein. The active site for aminoacyl-tRNA binding is highly conserved, while the active site for GTP/GDP binding lacks the cysteine present in the E. coli EF-Tu protein. The two introns separating exons 1 , 2 and 3 are, respectively, 103 and 110 nucleotides long. The size of the third intron is not yet determined. The splicing rules for eukaryote mRNA are not followed.


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