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Nucleic Acids Research, 1983, Vol. 11, No. 17 5893-5901
© 1983


CHEMISTRY

Chemical modification of cytosine residues of U6 snRNA with hydrogen sulfide (Nudeoddes and nudeotides. Part 49 [1])

Kazunobu Miura, Shigeki Tsuda, Fumio Harada+ and Tohru Ueda*

Faculty of Phannaceutical Sciences, Hokkaido University Kita-ku, Sapporo 060 +Virology Division, National Cancer Center Research Institute Tsukiji, Chuo-ku, Tokyo 104, Japan

*To whom correspondence should be addressed

Received July 11, 1983. Accepted August 8, 1983.

Sulfhydrolysis of cytosine residues to 4-thiouracil residues in mouse U6 snRNA was carried out to examine the secondary structure of U6 snRNA. The cytosine residues at positions 6, 42 and 68 were modified significantly, and at positions 11, 19 (or/and 25), 61 and 66 in moderate extent. Based on the result, the plausible secondary structure of U6 snRNA is discussed.


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D M Fortner, R G Troy, and D A Brow
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Genes & Dev., January 15, 1994; 8(2): 221 - 233.
[Abstract] [PDF]



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