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Nucleic Acids Research, 1983, Vol. 11, No. 17 5921-5940
© 1983

MOLECULAR BIOLOGY

Gene expression: chemical synthesis and molecular cloning of a bacteriophage T5 (T5P25) early promoter

Johanne Rommens, Douglas MacKnight, Lynn Pomeroy-aoney and Ernest Jay+

Department of Chemistry, University of New Brunswick Fredericton, New Brunswick E3B 6E2, Canada

+To whom correspondence should be addressed

Received June 22, 1983. Accepted August 9, 1983.

A sixty base pair DNA duplex containing the nucleotide sequence of the bacteriophage T5 early (T5P25) promoter has been constructed using a combination of chemical synthesis and enzymatic methods. Subsequent to cloning into pBR322, the promoter has been demonstrated to be biologically active being capable of directing the efficient expression of genes under its control. This serves as a prototype for an approach to the study of the iri vivo structure-function relationships and efficiency of promoters.


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