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Nucleic Acids Research, 1983, Vol. 11, No. 17 6065-6077
© 1983

MOLECULAR BIOLOGY

DNase I hypersensitivity in the {gamma} globin gene locus of K562 cells

Herbert M. Lachman and J.Gregory Mears+,*

Division of Experimental Hematology, Department of Medicine, Albert Einstein College of Medicine 1300 Morris Park Avenue, Bronx, NY 10461, USA

*To whom correspondence should be addressed

Received April 20, 1983. Revised August 1, 1983. Accepted August 1, 1983.

We have probed the chromatin conformation of the G{gamma}A'{gamma}'§ß globin gene locus of K562 cells, a human hematopoietic cell line, with the enzyme pancreatic DNAse I. This enzyme preferentially digests genes in an active configuration. We have found that in K562 cells, which produce embryonic and fetal but not adult hemoglobins, both the active {gamma} and inactive ß genes are DNAse I sensitive. However, only the active {gamma} genes have DNAse I hypersensitive regions. The hypersensitive regions have been mapped to an area approximately løø base pairs 5' to the G{gamma} and A{gamma} genes.

+Present address: Columbia University College of Physicians and Surgeons, Department of Medicine, Hammer Health Sciences Center, Room 1602, 701 West 168th Street, New York, Ny 10032, USA


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