Nucleic Acids Research, 1983, Vol. 11, No. 17 6079-6087
© 1983
MOLECULAR BIOLOGY |
The structure of cloned 3'-terminal RNA region of bovine leukemia virus (BLV)
*Institute of Microbiology Latvian SSR Academy of Sciences, Riga, USSR Institute of Organic Synthesis Latvian SSR Academy of Sciences, Riga, USSR
Received July 26, 1983. Accepted August 12, 1983.
cDNA synthesized on the bovine leukemia virus RNA template has been cloned in the pBR322 Pst I site. Colony hybridization with BLV RNA fragments and oligo(dT) has revealed a clone with cDNA insert containing 660 3'-terminal nucleoti-des of the BLV genome. The nucleotide sequence of the insert corresponding to U3 and R regions of the long terminal repeats (LTR) of viral genome has been determined. BLV U3, like U3 of other retroviruses, presumably contains promoter. The unusually long R region (about 230 bp), a certain homology with ATLV U3-R and some other structural features allow to group BLV LTR together with ATLV LTR in a separate class of retroviral LTR.