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Nucleic Acids Research, 1983, Vol. 11, No. 18 6185-6197
© 1983

MOLECULAR BIOLOGY

Hop stunt viroid: molecular cloning and nucleotide sequence of the complete cDNA copy

Takeshi Ohno*,+, Nobuhiko Takamatsu*, Tetsuo Meshi* and Yoshimi Okada*

*Department of Biophysics and Biochemistry, Faculty of Science, University of Tokyo Hongo, Tokyo 113 +Department of Agricultural Chemistry, Faculty of Agriculture, Tokyo University of Agriculture and Technology Fuchu-shi, Tokyo 183, Japan

Received July 28, 1983. Accepted September 5, 1983.

The complete cDNA of hop stunt viroid (HSV) has been cloned by the method of Okayama and Berg (Mol.Cell.Biol.2,161–170.(1982)) and the complete nucleotide sequence has been established. The covalently closed circular single-stranded HSV RNA consists of 297 nucleotides. The secondary structure predicted for HSV contains 67% of its residues base-paired. The native HSV can possess an extended rod-like structure characteristic of viroids previously established. The central region of the native HSV has a similar structure to the conserved region found in all viroids sequenced so far except for avocado sunblotch viroid. The sequence homologous to the 5'-end of Ula RNA is also found in the sequence of HSV but not in the central conserved region.


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