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Nucleic Acids Research, 1983, Vol. 11, No. 18 6243-6254
© 1983

MOLECULAR BIOLOGY

A novel approach to cloning transcriptionally active retrovirus-like genetic elements from mouse cells

Andrew T. Carter, John D. Norton and Roger J. Avery+

Department of Biological Sciences, University of Warwick Coventry CV4 7AL, UK +Department of Veterinary Science, Veterinary Research Laboratory, Montana State University Bozeman, MT 17, USA

Received July 19, 1983. Accepted August 18, 1983.

A family of dispersed, moderately repeated mouse genetic elements is expressedas retrovirus-like 30SRNA species (VL30 RNA) which can be transmitted to other cells when packagedas a pseudovirion complex by murine leukemia viruses (MuLV). Using the endogenous reverse transcriptase reaction of VL30 RNA-containing MuLV particles, full-length VL30 DNA was synthesized and cloned in pAT153. Analysis of a number of clones identified long terminal repeat structures (LTRs) characteristic of retrovirus proviruses and transposable genetic elements. Whilst the unique region of all clones was identical, the LTRs displayed some heterogeneity. Comparison of theunique region of cloned VL30 DNA with mouse genomic VL30 sequences showed the retrovirus-derived clones to be encodedby only a few membersof the divergent VL30 gene family. These findings thus demonstrate a method for cloning a defined sub-class of retrovirus-like cellular genes which are both transcriptionally active and transmissible by a retrovirus.


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