Nucleic Acids Research, 1983, Vol. 11, No. 18 6353-6368
© 1983
MOLECULAR BIOLOGY |
Possible Involvement of poly(A) in protein synthesks
Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School Worcester, MA 01605, USA
Received June 21, 1983. Revised August 30, 1983. Accepted August 30, 1983.
The experiments of this paper have re-evaluated the possibility that poly(A) is involved in protein synthesis by testing whether purified poly(A) might competitively inhibit in vitro protein synthesis in rabbit reticulocyte extracts. We have found that poly(A) inhibits the rate of translation of many different poly(A)+ mRNAs and that comparable inhibition is not observed with other ribopolymers. Inhibition by poly(A) preferentially affects the translation of adenylated mRNA5 and can be overcome by increased mRNA concentrations or by translating mRNPs instead of mRNA. The extent of inhibition is dependent on the size of the conipetitor poly(A) as well as on the translation activity which a lysate has for poly(A)+ RNA. In light of our results and numerous experiments in the literature, we propose that poly(A) has a function in protein synthesis and that any role in the determination of mRNA stability is indirect.
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