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Nucleic Acids Research, 1983, Vol. 11, No. 18 6487-6495
© 1983

MOLECULAR BIOLOGY

Primary structure of toe tolC gene that codes for an outer membrane protein of Escherichia coli K12

Jim Hackett and Peter Reeves

Department of Microbiology, University of Adelaide Adelaide, South Australia 5001, Australia

Received July 11, 1983. Revised August 16, 1983. Accepted August 16, 1983.

We present the nucleotide sequence of the tolC gene of Escherichia coli K12, and the amino acid sequence of the TolC protein (an outer membrane protein) as deduced from it. The mature TolC protein comprises 467 amino acid residues, and, as previously reported (1), a signal sequence of 22 amino acid residues is attached to the N-terminus. The C-terminus of the gene is followed by a stem-loop structure (8 base pair stem, 4 base loop) which may be a rho-independent termination signal. The codon usage of the gene is nonrandom; the major isoaccepting species of tRNA are preferentially utilised, or, among synonomous codons recognized by the same tRWA, those codons are used which can interact better with the anticodon (2,3). In contrast to the codon usage for other outer membrane proteins of E. coli (4) the rare arglnine codons AGA and AGG are used once and twice respectively.


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