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Nucleic Acids Research, 1983, Vol. 11, No. 18 6505-6512
© 1983

MOLECULAR BIOLOGY

Sequence for human argininosuccinate synthetase cDNA

Hans-Georg O. Bock*, Tsung-Sheng Su, William E. O'Brien and Arthur L. Beaudet

Department of Pediatrics and Cell Biology, Baylor College of Medicine Houston, TX 77030, USA

*To whom correspondence should be addressed

Received June 10, 1983. Revised August 31, 1983. Accepted August 31, 1983.

The nucleotide sequence for human argininosuccinate synthetase cDNA was determined by analysis of six clones isolated from a single experiment. The sequence covered 1623 nucleotides Including 76 bases of poly(A) and contained a 1236 nucleotide open reading frame encoding aprotein of 46,434 daltons. In one cDNA isolate,a cloning artifact or perhaps RHA polymerase error Involving addition of an A In a region of six A*prime;s within the coding sequence was documented. Single base variations in the 3'untranslated region were examined in detail since detection of DNA polymorphismsIn the cDNAs could imply over expression of both alleles at the active locus in canavanlne resistant cells, i.e.atrans acting mechanism for enzyme overproduction. However, the sequence from five cDNAs suggested some single base artifacts, and DNA polymorphism remains uncertain. The occurrence ofthree tandem arginine codone in the 5'untranslated region of the cDNA suggested the possibility of an Interaction of arginyl tRNA with mRNA to regulate RNA processingor half-life as a mechanism for arginlne-mediated repression.


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