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Nucleic Acids Research, 1983, Vol. 11, No. 19 6611-6629
© 1983

MOLECULAR BIOLOGY

Sequential stimulation of cellular RNA synthesis in polyoma-infected mouse kidney cell cultures

Jean-Marc Matter, Jean-Marie Tiercy and Roger Weil

Department of Molecular Biology, University of Geneva Geneva, Switzerland

Received August 4, 1983. Accepted September 16, 1983.

Lytic infection with polyoma virus leads in Go-arrested primary mouse kidney cell cultures to a mitotic host response. In the present work we focused our attention on cellular RNA synthesis shortly after onset of polyoma T-antigen synthesis. Onset of polyoma-induced stimulation of 45S pre-rRNA synthesis was determined by hybridization of total cellular RNA with a plasmid (pMrSalB) containing the 5'-end of the mouse ribosomal gene and of the other cellular RNA species by standard biochemical analysis of cellular fractions. The results showed that polyoma-induced stimulation of cellular hnRNA (hnRNP) synthesis, the earliest presently known host cell reaction, preceded onset of stimulated 45S pre-rRNA synthesis and that the latter was paralleled by polyoma-induced stimulation of 5S RNA, tRNA and overall protein synthesis. The polyoma-induced mitotic response is similar to that triggered by simian virus 40 and by certain nonviral mitogens.


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