Skip Navigation

This Article
Right arrow Print PDF (4548K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Knoll, B. J.
Right arrow Articles by O'Malley, B. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Knoll, B. J.
Right arrow Articles by O'Malley, B. W.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1983, Vol. 11, No. 19 6733-6754
© 1983

MOLECULAR BIOLOGY

Definition of the ovalbumin gene promoter by transfer of an ovalglobin fusion gene into cultured cells

Brian J. Knoll*, Tanya Zarucki-Schulz, Douglas C. Dean and Bert W. O'Malley+

Department of Cell Biology, Baylor College of Medicine Houston, TX 77030, USA

+To whom reprint requests should be sent

Received July 1, 1983. Revised August 31, 1983. Accepted August 31, 1983.

In order to study the initiation of transcription from the ovalbumin gene promoter, we constructed a hybrid gene (ovalglobin) in which 753 bps of ovalbumin gene 5'-flanking sequence were joined to the chicken adult ß-globin gene. When transfected into HeLa S3 cells, ovalglobin gene transcription initiated at the ovalbumin gene cap site, as measured by S1 nuclease and primer extension analysis. Deletion of 5'-flanking sequences to position –95 had little effect on transcription; deletion to –77 reduced transcription to about 20% of the wild type level and deletion to –48 reduced the level to about 2%. A deletion to –24, removing the sequence TATATAT, abolished transcription entirely. Hormonal regulation of the ovalglobin gene was observed when primary oviduct cells were used as recipients for DNA transfection. Under these conditions, addition of progesterone increased the level of ovalglobin transcripts to mere than 10 times the uninduced level.

*Present address: Department of Pathology and Laboratory Medicine, University of Texas Health Science Center at Houston, Houston, TX 77025, USA


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
R. J. Urban, M. Nagamani, and Y. Bodenburg
Tumor Necrosis Factor alpha Inhibits Transcriptional Activity of the Porcine P-45011A Insulin-like Growth Factor Response Element
J. Biol. Chem., December 6, 1996; 271(49): 31699 - 31703.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.