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Nucleic Acids Research, 1983, Vol. 11, No. 19 6787-6802
© 1983

MOLECULAR BIOLOGY

Nucleotides In 16S rRNA that are required in unmodified form for features recognized by ribosomal protein S8

David L. Thurlow*, Chantal Ehresmann and Bernard Ehresmann

Institut de Biologie Moleculaire et Cellulaire du CNRS 15 rue Rene Descartes, 67084 Strasbourg Cedex, France

Received June 2, 1983. Revised September 14, 1983. Accepted September 14, 1983.

Nucleotides in 16S rRNA which are required in unmodified form for specific recognition of ribosomal protein S8 from Escherichia coli were identified using a damage-selection experimental approach. Prior to complex formation with S8, 16S rRNA was treated under fully denaturing conditions with either diethyl pyrocarbonate or 25% hydrazine. Following separation of bound from unbound fragments of RNA, those associated with S8 were analyzed for their content of modified bases by treatment with aniline. Nucleotides found to be consistently unmodified in such fragments were located near the base of a stable helix (encompassing bases 581–656) or near the apex of the helix on the 3' proximal side. A minor S8 ribonucleoprotein particle was found to contain fragments which extended in the 3' direction to position 671.

*Present address: Department of Biochemistry, University of Massachusetts, Amherst, MA 01003, USA


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