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Nucleic Acids Research, 1983, Vol. 11, No. 2 251-264
© 1983

MOLECULAR BIOLOGY

A small DNA molecule containing covalently-linked ribonucleotides originates from the large intergenic region of the cauliflower mosaic virus genome

Simon N. Covey, David Turner and Gert Mulder

Department of Virus Research, John Innes Institute, Colney Lane Norwich NR4 7UH, UK

Received October 19, 1982. Revised December 13, 1982. Accepted December 13, 1982.

We have detected a small DNA molecule (sa-DNA), 725 nucleotides long, in cauliflower mosaic virus (CaMV)-infected, but not non-infected, turnip leaves. Alkali and RNase A treatments shortened as-DNA by 100 nuleotides and we conclude that it contains covalently-linked ribonucleotides. This DNA co-purified with cellular polyadenylated RNA. It is complementary to the ß-strand of CaMV DNA and of opposite polarity to RNAs transcribed from the {alpha}-strand. Hybridisation studies suggest that sa-DNA originates from the large intergenic region (IRI) of the CaMV genome. A small double-stranded DNA with three single-stranded components, which co-purifies with cellular DNA, appears to be related to sa-DNA but lacks detectable ribonucleotides.


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