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Nucleic Acids Research, 1983, Vol. 11, No. 2 321-338
© 1983

MOLECULAR BIOLOGY

Members of the Kpnl family of long interspersed repeated sequences join and interrupt {alpha}-satellite in the monkey genome

Giovanna Grimaldi and Maxine F. Singer

Laboratory of Biochemistry, National Cancer Institute, National Institutes of Health Building 37, Room 4E28, Bethesda, MD 20205, USA

Received October 5, 1982. Revised December 20, 1982. Accepted December 20, 1982.

Three different members of a family (KpnI-family) of interspersed repeated DNA sequences were found linked to {alpha}-satellite sequences in cloned segments of the African green monkey genome. In two of these segments the KpnI-family member is over 6 kbp in length and one of them is flanked by {alpha}-satellite on both sides indicating that it was inserted into a satellite array. Hybridization of subcloned portions of the family members to restriction endonuclease digests of monkey and human DNA and to a genomic library of African green monkey DNA indicate that 1) family members are interspersed in both the monkey and human genomes, 2) some family members may include sequences in addition to those in the three characterized here, 3) some family members may contain only parts of the sequences characterized here and 4) while the overall organization of the family is similar in the human and monkey genome the majority of the family members in each of the two genomes are distinctly identified by the variant position of certain restriction endonuclease sites. This last observation suggests that within each genome there is a tendency to maintain particular versions of the sequence. Observations 2) and 3) suggest that the KpnI family is complex and includes a variety of subfamilies.


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