Nucleic Acids Research, 1983, Vol. 11, No. 2 403-420
© 1983
MOLECULAR BIOLOGY |
Molecular cloning and analysis of the CRY1 gene: a yeast ribosomal protein gene
Department of Biological Sciences, Carnegie-Mellon University Pittsburgh, PA 15213, USA
*To whom reprint requestd should be addressed.
Received September 28, 1982. Revised December 15, 1982. Accepted December 15, 1982.
Using cloned DNA from the vicinity of the yeast mating type locus (MAT) as a probe, the wild type allele of the cryptopleurine resistance gene CRY1 has been isolated by the technique of chromosome walking and has been shown to be identical to the gene for ribosomal protein 59. A recessive cryRl allele has also been cloned, using the integration excision method. The genetic distance from MAT to CRY1 is 2.2 cM, while the physical distance is 21 kb, giving a ratio of about 10kb/cM for this interval. The phenotypic expression of both plasmid borne alleles of the gene can be detected in vivo. The use of this gene as a hybridization probe to examine RNA processing defects in the rna 2, rna 3, rna 4 , rna 8, and rna 11 mutants is also discussed.
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