Nucleic Acids Research, 1983, Vol. 11, No. 2 541-552
© 1983
MOLECULAR BIOLOGY |
Sequence organization in Dictyostelium: unique structure at the 5'-ends of protein coding genes
Department of Biology, B-022, University of California San Diego, La Jolla, CA 92093, USA
Received September 20, 1982. Revised December 29, 1982. Accepted December 29, 1982.
We have compared the sequences which lie 5' to the coding regions of 15 Dictyostelium genes transcribed by RNA polymerase 11. These sequences are extremely ({small tilde}90%) A + T rich and contain extensive homopolymeric regions. Like most eukaryotic genes, those in Dictyostelium possess a TATA or Goldberg-Hogness Box {small tilde}25-35bp 5' to the site of transcription initiation. In addition, each gene contains an oligo (dT) stretch between the TATA Box and mRNA start site; this oligo (dT) sequence is, thus far. unique to Dictyostelium. We suggest that the TATA-oligo (dT) structure is an essential component of the Dictyostelium promoter. The general sequence structure of coding, non-coding and untranscribed flanking regions in Dictyostelium is also discussed.
1Present address: Laboratory of cell and Developmental Biology, NIADDK(6/B1-12), National Institutes of Health, Bethesda, MD 20205, USA
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