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Nucleic Acids Research, 1983, Vol. 11, No. 20 6957-6964
© 1983

MOLECULAR BIOLOGY

The gene for the small subunit of ribulose-1,5-bisphosphate carboxyiase/oxygenase is located close to the gene for the large subunit in the cyanobacterium Anacystis nidulans 6301

Kazuo Shinozaki and Masahiro Sugiura

Department of Biology, Faculty of Science, Nagoya University Chikusa, Nagoya 464, Japan

Received August 26, 1983. Accepted September 26, 1983.

The gene for the small subunit (SS) of ribulose-1 ,5-bisphosphate carboxylase/oxygenase from a cyanobacterium, Anacystis nidulans 6301, has been cloned and subjected to sequence analysis. The SS coding region is located close to and downstream from the large subunit (LS) coding region on the same DNA strand. The spacer region between the LS and the SS coding regions contains 93 base pairs (bp), and has no promoter-like sequences. The coding region of A. nidulans SS gene contains 333 bp (111 codons). The deduced amino acid sequence of the A. nidulans SS protein shows 40% homology with those of higher plants.


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