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Nucleic Acids Research, 1983, Vol. 11, No. 20 6985-6996
© 1983

MOLECULAR BIOLOGY

Structure and function of an AT-rich, interspersed repetitive sequence from Chironomus thummi: solenoidal DNA, 142 bp palindrome-frame and homologies with the sequence for site-specific recombination of bacterial transposons

Norbert Israelewski*

Ruhr-Universität Bochum MA 5, Lehrstuhl für Genetik, D-4630 Bochum, FRG

Received July 19, 1983. Revised October 3, 1983. Accepted October 3, 1983.

Chironomus thummi thummi contains a repetitive AT-rich 118 bp sequence mainly in the centromere regions and elsewhere in the genome (1). A large cluster of repeats is regularly present in the non-transcribed spacer of rDNA. Dimers and multimers of the repeat migrate slower in small pore gels than would be expected from their size. The results indicate a solenoidal structure with a coil girth of appr. 350 bp. This structure is most probably due to a highly periodic positioning of di-nucleotides of the type purine - purine or pyrimidine-pyrimidine with distances of appr. 10 bases. In a cluster of 118 bp repeats, regions of dyad-symmetry are positioned such that a 142 ± 2 bp palindrome-frame is generated. Evidence is presented favouring the assumption that the repeat functions primarily in sister chromatid exchange.

*Dedicated to Prof. Dr. B. E. Wolf in honour of his 75th birthday on September 27, 1983


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