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Nucleic Acids Research, 1983, Vol. 11, No. 20 7057-7068
© 1983

MOLECULAR BIOLOGY

Different RNA patterns of globin and non-globin 40S heterogeneous nuclear RNA-protein complexes in chicken reticulocyte nuclei

Hai-Mei Huang+ and Chi-Bom Chae*

Department of Biochemistry, University of North Carolina Chapel Hill, NC 27514, USA

*To whom all correspondences should be addressed

Received June 23, 1983. Accepted September 15, 1983.

40s hetergeneous nuclear RNA-protein complex (HnRNP) was isolated from chicken reticulocyte nuclei after digestion of RNA by the endogenous nuclease. The size of the total RNA and ß globin RNA was compared by agarose and high resolution acrylamide gel electrophoresis. The results show that size of the total RNA ranges between 20 to 650 bases and the most prominent sizes are between 20 and 70–80 bases. The size of the RNA increases at roughly 10 base intervals between 40 and 70 bases on an acrylamide gel. The size of the ß globin RNA is smaller (25–50 bases) than the total RNA and also the RNA pattern is considerably different from that of total RNA. This suggests that, although the overall structure of 40s HnRNP might be the same, there is significant differences in the interaction of globin RNA with HnRNP protein and that of non-globin RNA with HnRNP protein in chicken reticulocyte nuclei.

+Present address: Dept. of Microbiology and Immunology, New York Medical College, Valhalla, NY 10595.


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