Skip Navigation

This Article
Right arrow Print PDF (607K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (32)
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Potter, B. V.L.
Right arrow Articles by Uznanski, B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Potter, B. V.L.
Right arrow Articles by Uznanski, B.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1983, Vol. 11, No. 20 7087-7103
© 1983

MOLECULAR BIOLOGY

A stereospeifically 18O-labelled deoxydinucleoside phosphate block for incorporation into an oligonucleotide

Barry V.L. Potter, Fritz Eckstein* and Bogdan Uznanski

Max-Planck-Institut für experimentelle Medizin, Abteilung Chemie Hermann-Rein-Str. 3, D-3400 Göttingen, FRG Polish Academy of Sciences, Centre for Molecular and Macromolecular Studies, Department of Bioorganic Chemistry 90-362 Lodz, Boczna 5, Poland

*To whom reprint requests should be addressed

Received September 14, 1983. Accepted September 29, 1983.

Fully protected diastereoisomers of deoxyguanylyl(3' -> 5')deoxyadenosine stereospecifically labelled on phosphorus with oxygen-18 have been synthesized by oxidation of phosphite triester intermediates in the presence of 18O-labelled water. The diastereoisomers have been chromatographically separated and their absolute configuration at phosphorus determined. (Rp)-[18o]deoxyguanylyl(3' -> 5') deoxyadenosine has been prepared by complete deprotection of the parent diastereoisomer of the Sp configuration. Methylation of the former compound permits assignment of the absolute configurations of the methyl esters of N1-methyldeoxyguanylyl(3' -> 5')N1-methyldeoxyadenosine.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.