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Nucleic Acids Research, 1983, Vol. 11, No. 21 7303-7316
© 1983

MOLECULAR BIOLOGY

The D-JH complex is an intermediate to the complete immunoglobulin beavy-chain V-region gene

Yoshio Yaoita, Norisada Matsunami, Cha Yong Choi, Haruo Sugiyama+, Tadamitsu Kishimoto+,* and Tasuku Honjo

Department of Genetics Kita-ku, Osaka 530, Japan +Internal Medicine, Osaka University Medical School Kita-ku, Osaka 530, Japan

*Present address: Institute for Molecular and Cellular Biology, Osaka University, Osaka 530, Japan

Received September 21, 1983. Accepted October 11, 1983.

We have examined the organization of the immunoglobulin JH segments in three clones derived from a single Abelson murine leukemia virus-transformed cell. Cloning and nucleotide sequence analyses of the JH-containing fragments have revealed the rearrangement from the preformed D-JH complex to the complete VH-D-JH gene, which was accompanied by the expression of the intra-cytoplasmic V chain. In one case a JH segment downstream to the preformed D-JH was used to create a new VH-D-JH gene. Upon the D-JH and Vh-D-JH rearrangements the intervening D segments were deleted from the chromosome. Ore of the expressed VH genes suffered from a large deletion of the 3' portion (including the 95th cysteine residue) of the VH segment. He discuss the possible mechanism of the allelic exclusion.


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