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Nucleic Acids Research, 1983, Vol. 11, No. 21 7375-7385
© 1983

MOLECULAR BIOLOGY

Isolation and translation of mRNA coding for the variant surface antigens of Paramecium

John Sommerville

Department of Zoology, University of St. Andrews SL Andrews, Fife KY16 9TS, UK

Received August 25, 1983. Revised October 14, 1983. Accepted October 14, 1983.

In the poly(A)+ RNA isolated from the ciliate Paramecium primaurelia is found a discrete and abundant mRNA species of high molecular weight (corresponding to about 9000 nucleotides).This mRNA species has size and abundance characteristics that identify it tentatively as the message coding for the variant cell-surface antigens. After microinjection of the high molecular weight mRNA into amphibian oocytes, polypeptides are synthesized that are immunoprecipit-ated specifically with antibodies directed against the homologous Paramecium antigen. On collecting the culture medium of oocytes ndcroinjected with Paramecium mRNA, newly-synthesized complete antigen molecules (Mr {bsim}300,000) can be recovered by immunoprecipitation.


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