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Nucleic Acids Research, 1983, Vol. 11, No. 21 7427-7434
© 1983

MOLECULAR BIOLOGY

Isolation and characterisation of cDNA clones for the A{alpha}- and {gamma}-chains of human fibrinogen

A.M.A. Imain, M.A.W. Eaton1, R. Williamson and S. Humphries

Department of Biochemistry, St.Mary's Hospital Medical School, University of London London W2 1PG 1Department of Chemistry Cdltech Ltd., 250 Bath Road, Slough SL1 4DY, UK

Received July 26, 1983. Revised October 13, 1983. Accepted October 13, 1983.

cDNA clones coding for the A{alpha}- and {gamma}-chains of human fibrinogen have been isolated from an adult liver cDNA library. Clones were identified by hybridisation with mixtures of synthetic oligonucleotides 17 bases long, predicted using amino acid sequence data for each chain. The cDNA insert sizes are l,950bp for A{alpha}( -fibrinogen and 950bp for {gamma}-tibrinogen. The clones do not show any cross-hybridisation. Each cDNA hybridises to a unique sequence in the human genome. In adult human liver. Northern blots give an estimated messenger RKA size of 2.6kb for A{alpha}-fibrinogen and 1.8kb for {gamma}-fibrinogen.


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