Nucleic Acids Research, 1983, Vol. 11, No. 21 7631-7648
© 1983
MOLECULAR BIOLOGY |
Expression of recombinant plasmids in mammalian cells Is enhanced by sodium butyrate
Laboratory of Molecular Biology, National Cancer Institute, Division of Cancer Biology and Diagnosis, National Institutes of Health Bethesda, MD 20205 Program in Biochemistry and Biophysics, Washington State University Pullman, WA 99164, USA
+Present Address: Eukaryotic Molecular Biology Group, Cancer Research Campaign, Biochemistry, Imperial College of Science and Technology, London SW7 2AZ, UK
Received July 1, 1983. Revised September 30, 1983. Accepted September 30, 1983.
We have studied the effects of sodium butyrate on DNA-mediated gene transfer in an effort to investigate interrelationships between chromatin structure and expression of recombinant plasmids. Our results demonstrate that butyrate affects the early stages of gene activity following DNA uptake at least two levels. First, the number of cells able to express foreign DNA increases from 10% to up to 40%. Second, there is an increase in enhancer-dependent transcription, approximately 30 fold in HeLa cells, involving the SV40 early promoter. Stable transformation efficiencies increase to 4% and 10% in HeLa S3 and monkey kidney CV-1 cells, respectively. Finally, expression of Integrated recombinant plasmid genes is reinducible by a second treatment five weeks after Initial
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