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Nucleic Acids Research, 1983, Vol. 11, No. 22 7717-7733
© 1983

MOLECULAR BIOLOGY

Transcriptional activity of the human psendogene {Phi}{alpha} globin compared with {alpha} globin, its functional gene counterpart

E. Whitelaw and N.J. Proudfoot

Sir William Dunn School of Pathology, University of Oxford South Parks Road, Oxford 0X1 3RE, UK

Received September 27, 1983. Accepted October 28, 1983.

Transcriptional analysis of the human pseudogene {Phi}{alpha} globin has revealed the following features: (1) The promoter with a 23 bp deletion between the CCAAT and ATA boxes is functional both in vitro and in vivo, 3 fold and 10 fold less efficient, respectively, than {alpha}. (2) Both the {Phi} and {alpha} globin gene promoters are active in the absence of transcriptional enhancers, either a gene-encoded or viral enhancer. (3) The mutated poly(A) addition signal in {Phi}{alpha} (AATGAA) appears to be completely nonfunctional. This result provides an explanation for the absence of {Phi}{alpha} transcripts in human erythroid cells.


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