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Nucleic Acids Research, 1983, Vol. 11, No. 22 7795-7817
© 1983

MOLECULAR BIOLOGY

Sequence analysis of 28S ribosomal DNA from the amphibian Xenopus laevis

Vassie C. Ware, Brian W. Tague*, C. Graham Clark**, Richard L. Gourse+, Reindert C. Brand++ and Susan A. Gerbi

Division of Biology and Medicine, Brown University Providence, RI 02912, USA

*Department of Biology, University of California-San Diego, La Jolla, California

**The Rockefeller University, New York, New York 10021

+Institute for Enzyme Research, University of Wisconsin, Madison, Wisconsin 53706

++Genetisch Laboratorium, Katholieke Universiteit, Nijmegen, The Netherlands

Received August 29, 1983. Revised October 19, 1983. Accepted October 19, 1983.

We have determined the complete nucleotide sequence of Xenopus laevie 28S rDNA (4110 bp). In order to locate evolutionarily conserved regions within rDNA, we compared the Xenopus 28S sequence to homologous rDNA sequences from yeast, Physarum. and E. coli. Numerous regions of sequence homology are dispersed throughout the entire length of rDNA from all four organisms. These conserved regions have a higher A+T base composition than the remainder of the rDNA. The Xenopus 28S rDNA has nine major areas of sequence inserted when compared to E. coli 23S rDNA. The total base composition of these inserts in Xenopus is 83Z G+C, and is generally responsible for the high (66Z) G+C content of Xenopus 28S rDNA as a whole. Although the length of the inserted sequences varies, the inserts are found in the same relative positions in yeast 26S, Physarum 26S, and Xenopus 28S rDNAs. In one insert there are 25 bases completely conserved between the various eukaryotes, suggesting that this area is important for eukaryotic ribosomes. The other inserts differ in sequence between species and may or may not play a functional role.


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