Skip Navigation

This Article
Right arrow Print PDF (3811K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Kunkel, L.M.
Right arrow Articles by Latt, S.A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kunkel, L.M.
Right arrow Articles by Latt, S.A.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1983, Vol. 11, No. 22 7961-7979
© 1983

MOLECULAR BIOLOGY

Identification and isolation of transcribed human X chromosome DNA sequences

L.M. Kunkel*, U. Tantravahi*,+, D.M. Kurnit*, M. Eisenhard*, G.P. Bruns* and S.A. Latt*,+

*Genctics Division, and Mental Retardation Center The Children's Hospital, Harvard Medical School, Boston, MA 02115, USA +Cytogenetks Laboratory Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA

Received June 30, 1983. Revised October 11, 1983. Accepted October 11, 1983.

A human X chromosome specific phage library has been used as a source of X-specific genomic DNA clones which hybridize with cellular RNA. Random cDNA clones were mapped for X chromosome sequence localization and 8 were identified as hybridizing to X chromosome Hind III fragments. All eight also hybridized with autosomal Hind III fragments. The X chromosome genomic sequences corresponding to two of these cDNA clones were isolated from a phage library constructed with the Hind III endonuclease digest products of X enriched DNA. One genomic DNA segment, localized to the short arm of the X, shared sequence homology with at least one region of the human Y chromosome. The methodology developed represents a rapid means to obtain a specific genomic DNA clone from a single chromosome when multiple different genomic loci homologous to an expressed DNA sequence exist.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 ScienceHome page
C. Chang, J Kokontis, and S. Liao
Molecular cloning of human and rat complementary DNA encoding androgen receptors
Science, April 15, 1988; 240(4850): 324 - 326.
[Abstract] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.