Nucleic Acids Research, 1983, Vol. 11, No. 22 8051-8061
© 1983
MOLECULAR BIOLOGY |
Nucleotide sequence encoding the precursor of the small subunit of ribulose 1,5-bisphosphale carboxylase from Lemna gibba L.G-3
Biology Department and Molecular Biology Institute, University of California Los Angeles, CA 90024, USA
*Present address: Laboratory for Molecular Biology, Agricultural University, 6703 BC Wageningen, The Netherlands
+To whom correspondence should be addressed
Received August 26, 1983. Revised October 20, 1983. Accepted October 20, 1983.
We have sequenced a cDNA clone, pLgSSU, which encodes the small subunit of ribulose l,5-b1sphosphate carboxylase of Lernna gibba L.G-3 a monocot plant. This clone contains a 832 basepair insert which encodes the entire 120 amino adds of the mature small subunit polypeptide (Mr = 14,127). In addition this clone encodes 53 amino acids of the amino terminal transit peptide of the precursor polypeptide and 242 nucleotides of the 31 non-coding region. Comparison of the nucl eoti de sequence of pLgSSU with Lemna gibba genomic sequences homologous to the 51 end of the cDNA clone suggests that nucleotides encoding four ami no-terminal amino adds of the transit peptide are not included 1n the cDNA clone. The deduced amino acid sequence of the Lemna gibba mature small subunit polypeptide shows 70–75% homology to the reported sequences of other species. The transit peptide amino add sequence shows less homology to other species. There is 50% homology to the reported soybean sequence and only 25% homology to the transit sequence of another monocot, wheat.