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Nucleic Acids Research, 1983, Vol. 11, No. 23 8237-8251
© 1983

MOLECULAR BIOLOGY

Nucleotide sequence and stability of the RNA component of RNase P from a temperature-sensitive mutant of E. coli

Hiroshi Sakamoto, Naohiro Kimura, Fumikiyo Nagawa* and Yoshiro Shimura

Department of Biophysics, Faculty of Science, Kyoto University Kyoto 606, Japan

Received September 14, 1983. Accepted October 25, 1983.

The gene coding for the RNA component of RNase P was cloned from a temperature-sensitive mutant of Escherichia coli defective in RNase P activity (ts7O9) and its parental wild-type strain (4273), and the complete nucleotide sequences of the gene and its flanking regions were determined. The 5'- and 3'-terminal sequences of the RNA component were determined and mapped on the DNA sequence. The mutant gene has GC-to-AT substitutions at positions corresponding to 89 and 365 nucleotides downstream from the 5' terminus of the RNA sequence. Comparing to the wild-type RNA, the mutant RNA is less stable and rapidly degraded in vivo and in vitro.

*Present address: Central Research Institute, Wakunaga Pharmaeutical Co., Koda-cho, Hiroshima 729-64.


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