Skip Navigation

This Article
Right arrow Print PDF (2521K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (30)
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Edwards, J. C.
Right arrow Articles by Tabak, H. F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Edwards, J. C.
Right arrow Articles by Tabak, H. F.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1983, Vol. 11, No. 23 8269-8282
© 1983

MOLECULAR BIOLOGY

Initiation of transcription of the yeast mitochondrial gene coding for ATPase subunit 9

John C. Edwards*, Klaas A. Osinga+, Thomas Christianson*, Lambert A.M. Hensgens+, Pim M. Janssens+, Murray Rabinowitz* and Henk F. Tabak+

*Departments of Medicine, Biochemistry and Biology, University of Chicago Chicago, IL 60637, USA +Section for Molecular Biology, Laboratory of Biochemistry, University of Amsterdam Kruislaan 318, 1098 SM Amsterdam, The Netherlands

Received September 6, 1983. Revised October 19, 1983. Accepted October 19, 1983.

We have determined transcriptional initiation sites for the ATPase subunit 9 gene on the yeast mitochondrial genome. Using S1 nuclease mapping, in vitro capping of primary transcripts with GTP and guanylyl transferase, and in vitro transcription analysis with purified mitochondrial RNA polymerase, we find the major site of transcriptional initiation to be at a point 630 nucleotides upstream of the coding region for the gene. In addition, we find much lower levels of initiation at a second site 78 nucleotides downstream of the first. Both initiation sites occur at the same position within a nonanucleotide sequence which we have previously found associated with initiation of rRNA synthesis. This work further supports the notion that this nonanucleotide sequence 1s an integral component of mitochondrial promoters and indicates that the same RNA polymerase is used for transcription of both mRNA and rRNA in yeast mitochondria.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.