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Nucleic Acids Research, 1983, Vol. 11, No. 23 8369-8387
© 1983

CHEMISTRY

Rapid synthesis of long-chain deoxyribooligonucleotides by the N-methylimidazolide phosphotriester method

V.A. Efimov, A.A. Buryakova, S.V. Reverdatto, O.G. Chakhmakhcheva* and Yu.A. Ovchinnikov

Shemyakin Institute of Bioorganic Chemistry, USSR Academy of Sciences Moscow 117988, USSR

*To whom reprint request should be sent

Received October 20, 1983. Accepted November 2, 1983.

A modified phosphotriester method has been employed for the efficient chemical synthesis of long-chain deoxyribooligonucleotides. During the course of this work, a general and rapid procedure was developed for the preparation of 24–62-mers in solution. Preparative reversed phase column chromatography on silanized silica gel was used to purify triester intermediates starting from 10-mers. The rapid synthesis of 32-mar and 42-mer on glass and silica gel supports using suitably protected 2–8-mer blocks as coupling units has been also accomplished. In particular, a convenient procedure for the solid-phase synthesis of oligonucleotide blocks bearing 3'-terminal phosphodiester groups is described.


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