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Nucleic Acids Research, 1983, Vol. 11, No. 23 8389-8405
© 1983

CHEMISTRY

Synthesis of short RNA fragments by the benzotriazolyl phosphotriester approach

C.T.J. Wreesmann, A. Fidder, G.A. van der Marel and J.H. van Boom*

Gorlaeus Laboratory, P.O. Box 9502, 2300 RA Leiden, The Netherlands

*To whom correspondence should be addressed

Received October 18, 1983. Accepted November 7, 1983.

Analytical and experimental evidence will be presented to show that addition of a slight excess of 2-chlorophenyl-0,0-bis[1-benzotriazolyl]phosphate to a properly 2',5'-protected ribonucleoside gives an intermediate 3'-phosphotriester, which, after addition of a 2'-protected ribonucleoside, affords a dimer containing solely a 3'-5'-internucleotide linkage. The above phosphorylation procedure has been used in the synthesis of the pentamer UpApCpGpC.


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