Skip Navigation

This Article
Right arrow Print PDF (4867K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Unnasch, T.R.
Right arrow Articles by Wirth, D.F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Unnasch, T.R.
Right arrow Articles by Wirth, D.F.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1983, Vol. 11, No. 23 8443-8459
© 1983

MOLECULAR BIOLOGY

The avian malaria Plasmodium lophurae has a small number of heterogeneous ribosomal RNA genes

T.R. Unnasch and D.F. Wirth

Department of Tropical Public Health, Harvard University Boston, MA 02115, USA

Received June 7, 1983. Revised November 1, 1983. Accepted November 1, 1983.

The structure and number of the ribosomal RNA (rRNA) genes of the avian malaria parasite Plasmodium lophurae has been examined using Southern blot analysis and recombinant DMA techniques. The ribosomal DNA (rDNA) of P. lophurae has been cloned Into the plasmid pBR322, beginning with size-selected populations of Cla I- and Hind III-restricted parasite DNA. The structure of two clones (CL-1 and HA-2) 1s presented in detail. These two clones together probably represent the entire 17s and 25s coding regions of P. lophurae.

Analysis of quantitative genomic Southern blots reveals that there are approximately six rRNA genes per haploid genome, and that the rRNA genes may be divided Into four distinct classes by restriction analysis. Examination of the flanking regions of these genes Indicates that they are not organized into easily recognizable tandem repeats.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 ScienceHome page
J. Gunderson, M. Sogin, G Wollett, M Hollingdale, V. de la Cruz, A. Waters, and T. McCutchan
Structurally distinct, stage-specific ribosomes occur in Plasmodium
Science, November 13, 1987; 238(4829): 933 - 937.
[Abstract] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.