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Nucleic Acids Research, 1983, Vol. 11, No. 23 8495-8508
© 1983

MOLECULAR BIOLOGY

A chicken repetitive DNA sequence that is highly sensitive to single-strand specific endonucleases

Kevin Dybvig*, Christy D. Clark, Giuseppi Aliperti+ and Milton J. Schlesinger

Department of Microbiology and Immunology, Washington University School of Medicine St. Louis, MO 63110, USA

Received August 18, 1983. Revised November 3, 1983. Accepted November 3, 1983.

A DNA sequence consisting of the 5-mer AGAGG repeated tandemly 32 times has been detected in a chicken genome clone and found to be present in about 2000 copies per chicken genome. This sequence was highly susceptible to single-strand specific endonucleases isolated from Aspergillus oryzae (S1) and mung bean, but cleavage by a single-strand specific endonuclease isolated from Neurospora crassa occurred only at a pH below 5.5. Endonucleolytic cutting of the AGAGG sequence by the single-strand specific enzymes required a supercoiled substrate and was independent of ionic strength.

*Present address: Department of Microbiology, University of Rochester Medical Center, Rochester, NY 14627, USA.

+Present address: Department of Medicine, St. Mary's Hospital, St. Louis, MO, USA.


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