Skip Navigation

This Article
Right arrow Print PDF (2437K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Blum, B.
Right arrow Articles by Vogt, V.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Blum, B.
Right arrow Articles by Vogt, V.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1983, Vol. 11, No. 23 8519-8533
© 1983

MOLECULAR BIOLOGY

Localization and DNA sequence around the initiation site of ribosomal RNA transcription in Physarum polycephalum

Beat Blum1, Thomas Seebeck1, Richard Braun1, Patrick Ferris2 and Volker Vogt2

1Institut für Allgemeine Mikrobiologie, University of Bern Baltzerstrasse 4, 3012 Bern, Switzerland 2Section of Biochemistry, Molecular and Cell Biology, Cornell University Ithaca, NY 14853, USA

Received May 17, 1983. Revised October 26, 1983. Accepted October 26, 1983.

We have used S1 nuclease to map the initiation site of ribosomal RNA transcription in the acellular slime mold Physarum polycephalum, and we have determined the sequence of 1011 nucleotides surrounding the start site. Consistent with others' observations, there is little homology with the comparable region of other species. As predicted by previous restriction mapping, direct repeats roughly 30 base pairs in length are present upstream of the initiation site and a 148 base pair duplication occurs in the external transcribed spacer. The results also suggest the presence of a processing site within the external transcribed spacer of the ribosomal transcription unit.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.