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Nucleic Acids Research, 1983, Vol. 11, No. 24 8555-8568
© 1983

MOLECULAR BIOLOGY

Nucleotide sequence of the transcriptional initiation region of the yeast GAL7 gene

Yasuhisa Nogi and Toshio Fukasawa

Laboratory of Molecular Genetics, Keio University School of Medicine Shinjuku-ku, Tokyo 160, Japan

Received October 25, 1983. Accepted November 21, 1983.

The GAL7 gene of Saccharomyces cerevisiae encodes Gal-1-P uridylyl transferase, the second enzyme of Leloir pathway for the galactose catabolism. We have determined the sequence of 1003 base pairs surrounding and upstream of the transcriptional initiation site of the GRL7 gene. The region sequenced also encompasses the 3' end of GAL10 gene. The 5' end of GAL7 mRNA was determined on the DNA sequence by the S1 nuclease- and exonuclease VII mapping, which is located 21 to 22 base pairs upstream from the translation initiating ATG codon. The primary structure of the GAL7 5' flanking region has many features common to those of multicellular eukaryotic genes. The 3' end of GAL10 mRNA was also determined by the mapping technique with the single-strand specific nucleases to be about 600 base pairs upstream from the 5; end of GAL7 mRNA.


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